The study of the effect and safety of homoharringtonine in the prevention of postlaminectomy epidural adhesion in rats

English Keywords:Laminectomy  Rat  Homoharringtonine  Epidural adhesion  Safety

English Abstract:

  【Abstract】 Objectives: To determine the effect of local application of the homoharringtonine(HHT) in preventing postlaminectomy epidural adhesion in rats. Methods: A total of 36 adult male SD rats, aged 2 months and weight 279.42±32.58g(260-320g), were selected and randomly divided into group A(HHT 0.2mg/ml group), group B(HHT 0.1mg/ml group), and group C(normal saline group). The L1 laminectomy model was made and the dural membrane was exposed. In group C, the wound was closed layer by layer after normal saline irrigation. In groups A and B, cotton pads with different concentrations of HHT were soaked into the bone defect area for 5min, and then the wound was closed layer by layer after normal saline irrigation. At 1 month after surgery, gross specimens and histopathology(HE staining) were used to observe the condition of epidural scar adhesion, and the fibroblast count and collagen optical density were analyzed by Image Pro Plus. Results: There were no obvious adhesions in group A, mild adhesions in group B, and tight epidural scar adhesions in group C. The collagen optical density and fibroblast count in epidural scar in group A was 0.063±0.014 and 8.43±2.52 respectively, which were significantly lower than that in group C(0.186±0.005 and 22.47±2.37), and were better than that in group B(0.126±0.017 and 14.60±1.67)(P<0.05). Conclusions: Topical application of homoharringtonine has a good effect in preventing postlaminectomy epidural adhesion.