Relationship between diffusion tensor imaging coefficient and expression of platelet activating factor in rabbits with acute spinal cord injury

English Keywords:Acute spinal cord injury  Diffusion tensor imaging  Platelet activating factor  Model  Rabbit

English Abstract:

  【Abstract】 Objectives: To analyze the correlation between the parameters including fractional anisotropy(FA) and apparent diffusion coefficient(ADC) of magnetic resonance diffusion tensor imaging and the content of platelet activating factor(PAF), and to explore the value of DTI in the diagnosis of acute spinal cord injury. Methods: 12 Chinese white rabbits, 6 were randomly selected as experimental group, and the other 6 as control group. Resection of T10 lamina on the experimental group was performed, then the Allen′s method was used to simulate the model of acute spinal cord injury, while the control group was only exposed the spinal cord, and dura mater remained intact. At each time point after modeling, the regular MRI and DTI scans were respectively performed, then the PAF concentrations in cerebrospinal fluid and femoral vein serum were tested. 3D Neuro software was used to measure FA value and ADC value on the FA chart and ADC chart, the FA value and the ADC value of the experimental group were analyzed by Pearson correlation with PAF content. Results: Compared with the control group, the ADC value at each time point significantly increased in the experimental group, the FA value was significantly lower(P<0.01). With the passage of time, the ADC value of the experimental group gradually increased, the FA value gradually decreased(P<0.01), there was no significant change in the control group at each time point(P>0.05). The plasma and cerebrospinal fluid PAF of experimental group were significantly higher than those of the control group(P<0.01), the plasma and cerebrospinal fluid PAF content of experimental group increased at 1d, 3d and 5d(P<0.01), but decreased at 7d(P<0.01), PAF values of the control group showed no significant change at each time point(P>0.05). Serum PAF and cerebrospinal fluid concentrations of the experimental group after modeling were positively correlated with ADC value at 1d, 3d, 5d, negatively correlated with ADC value at 7d(|r|>0.8, P<0.05), and it was negatively correlated with FA value at 1d, 3d, 5d, positively correlated with FA value at 7d(|r|>0.8, P<0.05). Conclusions: FA value and ADC value have a good correlation with PAF; DTI parameters can reflect the occurrence and development of secondary injury of spinal cord.