Association between RANK gene polymorphism and the occurrence of female adolescent idiopathic scoliosis

English Keywords:Adolescent idiopathic scoliosis  Receptor activator of nuclear factor-κB  Body mass index  Cobb anlge  Female

English Abstract:

  【Abstract】 Objectives: To study whether the receptor activator of nuclear factor-κB (RANK) gene polymorphism is associated with the occurrence of adolescent idiopathic scoliosis(AIS) girls. Methods: 450 AIS girls and 400 age-matched healthy girls from Februry 2009 to July 2013 were recruited in this study, the average age was 14.8±2.9 years(range, 10-18 years) for AIS girls and 15.1±2.3 years(range, 10-17 years) for healthy controls, respectively. Only AIS girls with Cobb angles larger than 20° were included in this study. The anthropometric parameters including height, weight and body mass index(BMI) were available for all AIS girls. 2ml blood were taken from the antecubital vein in both groups and the DNA of peripheral leukocyte was extracted for each individual. The rs1805034 and rs35211496 were selected as target single nucleotide polymorphism(SNP) to cover all of the polymorphisms on RANK. Genotyping was performed with fluorescent quantitative polymerase chain reaction(PCR). Comparison of genotyping results between 2 groups were performed by using the χ2 while one-way ANOVA tests to compare the Cobb angle and BMI among AIS girls with different genotypes. Results: The rs1805034 and rs35211496 were successfully genotyped, of which no significant difference of genotype frequencies from the Hardy-Weinberg equilibrium test was noted for the AIS patients or the normal controls(P>0.05). There was no significant difference of genotype and allele frequencies of rs1805034 and rs35211496 between the AIS girls and normal controls(P>0.05). By one-way ANOVA test, the Cobb angle and BMI showed no significant difference among different genotypes in rs1805034 and rs35211496(P>0.05). Conclusions: The RANK gene may be not the susceptibility gene of AIS girls. There is no significant difference among different genotypes in terms of Cobb angle and BMI in rs1805034 and rs35211496.