Expression of heat shock cognate protein 70 after spinal cord ischemia-reperfusion injury

English Keywords:Spinal cord ischemia-reperfusion injury  Heat shock cognate protein 70  Stress

English Abstract:

  【Abstract】 Objectives: To investigate the significance and variation of heat shock cognate protein 70(HSC70) expression after spinal cord ischemia-reperfusion injury. Methods: Thirty-six New Zealand white rabbits were randomly divided into six groups, and established SCIRI models by using the Zivin′s method. In group A, the abdominal aorta was revealed without blood blocked, marrow was obtained after abdominal closure. In group B, blood in abdominal aorta was blocked for 30 minutes before abdominal closure, then marrow was obtained. In the ischemia-reperfusion groups(C, D, E, F), blood was blocked for 30 minutes before reperfusion, motor function was evaluated by modification Tarlov score at 6h, 12h, 24h, 48h after reperfusion, then marrow was taken in each group. Adopted two-dimensional fluorescence difference gel electrophoresis to obtain the HSC70. Immunohistochemistry and Western blot were employed to analyze the role of HSC70 in SCIRI and provide theoretical evidence for the prevention, diagnosis and treatment of SCIRI. Results: SCIRI rabbits were set up successfully. The modified Tarlov scores at group C, D, E, F was 1.167±0.753, 1.667±0.516, 2.668±0.516 and 2.167±0.752 respectively. The function of experimental animals′ hind legs was gradually improved after SCIRI and reached the early best level after 24 hours reperfusion, while declined slightly after 48 hours reperfusion. The immunblot gray scale of HSC70 was clear in group A, strengthened slightly in group B, strengthened again in group C and D. But gray scale of group E decreased to the lowest, and that of group F returned to the level of 6~12 hours reperfusion. Small interneurons cytoplasm of central gray matter in group A had mild immune response; the cytoplasm of neurons of grey matter in group B had the same immune responses as group A; immune response of glial cells nucleus in group C was aggravated; immune response of neurons in group D decreased, but that of glial cells and Schwann cells nucleus was strong; immune response of neurons in group E was further weakened, and below the level of group A; immune response of neuronal cytoplasm in group F was aggravated, while that of glial cells and Schwann cells nucleus was still strong. Conclusions: HSC70 involves in the occurrence and development of SCIRI and it can be the target of prevention, diagnosis and treatment of SCIRI.