The experimental study of sustained drug release of calcium sulfate/amino acid polymer artificial bone carrying 3 anti-TB drugs: an in vitro study

English Keywords:Anti-TB drugs  Calcium sulfate/amino acid polymer artificial  Sustained-release  In vitro

English Abstract:

  【Abstract】 Objectives: To explore the properties of sustained drug release of the calcium sulfate/amino acid polymer artificial bone carrying 3 anti-TB in vitro. Methods: The calcium sulfate/amino acid polymer artificial bone carrying 3 anti-TB drugs was produced in dark environment[proportion: 100∶3∶3∶12 calcium sulfate/amino acid composite 500mg, isoniazid(INH) 15mg, rifampicin(RFP) 15mg, pyrazinamide(PZA) 60mg)]. The concentration of each drug(INH, RFP, PZA) was detected by high performance liquid chromatography(HPLC) and the unit period of drug release quality at 3h, 12h, 24h, 36h, 48h, 60h, 72h, 1-14w was measured, respectively in simulated body fluid in vitro. Results: The concentration of INH, RFP and PZA release in simulated body fluid of triple anti-tuberculosis drugs calcium sulfate/poly(amino acids) bone at 3h reached 152.96±1.32μg/ml，92.90±2.17μg/ml and 334.90±12.3μg/ml, respectively, and the three kinds of drug concentrations were higher at each time point before eight weeks. The concentration of PZA at the eighth week, the concentration of RFP at the 10th week and INH at the 11th week were still 10 times higher than the minimum inhibitory concentration(MIC), then decreased gradually. There was no contamination peak in drug release of effective measurement time in control group. Conclusions: Triple anti-tuberculosis drugs calcium sulfate/poly(amino acids) bone can reach reliable and longer duration of effective drug release. Concentrations of three drugs are high enough to kill mycobacterium tuberculosis otheoretically.