The effect of bipedal amputation on a scoliosis model of female mouse with low melatonin level

English Keywords:Scoliosis  Low melatonin level  Bipedal amputated female mice

English Abstract:

  【Abstract】 Objectives: To establish a better adolescent idiopathic scoliosis(AIS) model of bipedal amputated mouse with low melatonin level. Methods: Twenty C57BL/6j female mice and twenty male mice were chosen to serve as low melatonin and divided into two groups according to gender, and twenty C3He/ej male mice were treated as controls. Amputation of forelimbs and tail was performed at 3 weeks old in three groups. After amputation, all these bipedal amputated mice were trained to keep a standing posture. Then X-rays were obtained at the 4th month to determine the development of spinal deformity. Comparisons on the incidence of scoliosis, and curve magnitude were performed among groups. Results: After establishing models, all mice were capable of keeping a standing posture for a long time. Scoliosis was observed in 19 out of 20 C57BL/6j female mice with an average Cobb angle of 24.6°±6.0°(range, 17° to 36°), in 16 out of 20 C57BL/6j male mice with an average Cobb angle of 20.8°±4.3°(range, 13° to 31°) and in 9 out of 20 controls with an average Cobb angle of 18.3°±2.7°(range, 13° to 23°). Conclusions: A higher scoliosis incidence and more severe curve are observed in C57BL/6j female bipedal mice compared with C57BL/6j male bipedal mice and control mice, which indicates that this model can be used for the research of AIS.