Preparation and property of anti-tuberculosis drug carrying composite scaffold

English Keywords:Bone tuberculosis  Anti-TB drugs  Drug carriers  Poly(lactic-co-glycolic)  Tri-calcium phosphate

English Abstract:

  【Abstract】 Objectives: To prepare composite scaffold and investigate its drug-loaded property, drug delivery and biocompatibility. Methods: The porous poly(lactic-co-glycolic)/tri-calcium phosphate/anti-tuberculosis drug (INH) composite scaffold was prepared by combining particle leaching and phase separation technique. The morphology and the structure of scaffold were observed by SEM. The compressive strength and porosity were measured in vitro. INH release behavior, drug loading and encapsulation efficiency of scaffold were determined by ultraviolet spectrophotometry. The scaffolds were implanted into the SD rats, and gross observation and histology assay were conducted 4 weeks after operation. Results: It could be seen that the scaffold possessed a highly interconnected porous structure with the diameter of macro-pore about 150-300μm, and the average diameter was about 222±23μm, and a lot of micro-pores with diameter of about 10μm were found in the wall of intro-and inter-macro-pores. The porous composite scaffold possessed good physical properties with 1.93±0.65MPa compressive strength and (86±3)% porosity and (66.73±2.65)% encapsulation. Drug release process was stable in vitro and the release curve was smooth. Surrounding tissue was normal in vivo with no degeneration and necrosis of cells. Conclusions: The porous composite scaffold perceives good biomechanical properties, porosity, INH release and biocompatibility, which can be used as bone repair as well as anti-tuberculosis material after debridement.