The relationship between Id-1, VEGF as well as microvessel density and blood loss in spinal metasta?鄄sis surgery and tumor origin

English Keywords:Spinal metastasis  Id-1  VEGF  Microvessel density  Immunohistochemistry

English Abstract:

  【Abstract】 Objectives: To investigate the distributions of Id-1, vascular endothelial growth factor (VEGF)　and microvessel density (MVD) in metastatic spinal tumor with different origins and their association with intraoperative blood loss. Methods: 32 metastases spinal specimens confirmed by pathological method in our hospital from 2005 to 2008 were collected. Based on the amount of blood loss, all cases were divided into low, medium and high blood loss subgroup, and all specimens were divided into epithelial group and mesenchymal derived group based on the origins of metastases. Immunohistochemical staining was used to detect Id-1, VEGF and CD34(microvascular markers)　in all specimens. The above-mentioned index, tumor origin and blood loss were analyzed each other. Results: Id-1 associated significantly with tumor origin(P<0.05), and showed positive relationship with intraoperative blood loss; Metastases from different origins had different expressions of VEGF (P<0.05), which were positively correlated with the amount of blood loss; no significant difference was noted between MVD and metastases with different origins (P＞0.05). Spinal metastases with different origins showed statistical significance　with blood loss(P<0.05). Conclusions: Id-1 and VEGF expressions are different as for different metastasis origins, and are positively correlated with blood loss. Microvessel density is not significantly influenced by origin of spinal metastases. The epithelial origin metastases are associated with more blood loss.