Different doses of ｏlfactory ensheathing cells transplantation in subarachnoid for repairment of spinal cord injury, an experimental study

English Keywords:Olfactory ensheathing cells  Spinal cord injury  Subarachnoid transplantation  Dose

English Abstract:

  【Abstract】 Objective：To investigate the feasibility, safety and dose-effect relationship of olfactory ensheathing cell transplantation for spinal cord injury.Method：Rats with T10 spinal cord injury induced by modified Allen method were randomly divided into four groups and received immediate cell transplantation as followings：high-dose olfactory ensheathing cells（OECs）　transplantation in subarachnoid（group A），medium－dose transplantation（group B），low-dose transplantation（group C），control group with DMEM/F12 transplantation（group D）.The neurofunctional recovery was noted by combined behavioral scores（CBS）；ｔhe axons regeneration and the survival of olfactory ensheathing cells at the different time-point recorded either.Results：The neurofunction of group A and B improved significantly compared with group C or D according to combined behavioral scores（CBS），whereas no difference was noted between group A and B，as well as same as between group C and D.Ｉｔ　ｗａｓ　ｆｏｕｎｄ　ｂｙ　ｈｉｓｔｏｌｏｇｉｃａｌ　ｏｂｓｅｒｖａｔｉｏｎ　ｔｈａｔ the number of nerve fibers of group A and group B wａｓ more than group C and D.Conclusion：The OEC transplantation in subarachnoid for acute spinal cord injury is reliable, and high dose showｓ good prognosis.