Effect of stress on differential protein expression of fibroblast in postrerior longitudinal ligament of patients with ossification of cervical posterior longitudinal ligament

English Keywords:Ossification of posterior longitudinal ligament  Cervical  Fibroblast cells  Electrophoresis  Proteomics

English Abstract:

  【Abstrat】 Objective：To investigate the effect of stress on differential protein expression of fibroblast in postrerior longitudinal ligament of patients with ossification of cervical posterior longitudinal ligament（OPLL）and the inner mechanism.Method：Cervical posterior longitudinal ligaments were collected from three OPLL patients，and fibroblast was cultured by tissue fragment culturing method after removal of bone.Ligament fibroblast cells were subjected to mechanical strains using a Flexercell strain unit by 10%，0.5Hz for 24 hours，and the static cells were used as control.Protein lysates were extracted separately，and differential gel electrophoresis（DIGE） and MALDI-TOF/TOF mass spectrum was performed to identify the differential expression of proteins.According to papers published，the localization of the proteins，function and possible role in the developing of OPLL were reviewed.Result：15 differential expressed proteins were identified，among which 14 were down-regulated and 1 was up-regulated.The known differentially expressed proteins were classified as metabolic enzymes，translation related protein，cell cytoskeleton and other proteins，4 proteins played important role in the pathogenesis of ossification.Conclusion：Stress may induce differential expression of several ossification-related proteins in postrerior longitudinal ligament fibroblast，some of them may be involved in the developing of OPLL.