The dose-effect relationship and safety of topical mitomycin C in preventing postlaminectomy peridu?鄄ral adhesion in rats

English Keywords:Laminectomy  Peridural adhesion  Mitomycin C  Dose-effect relationship concentration  Security  Rat

English Abstract:

  【Abstract】 Objective：To investigate the dose-effect relationship and safety of topical mitomycin C（MMC） in preventing postlaminectomy peridural adhesion in rats.Method：Laminectomy was performed at L1 in 60 rats，cotton pads soaked either with 0.1mg/ml（group B），0.3mg/ml（group C），0.5mg/ml（group D），0.7mg/ml（group E） MMC，or saline（group A） were filled into the operative sites for five minutes.The latent period of the somatosensory evoked potential（SEP） before and after administration in each group was tested．The rats were sacrificed 4 weeks after surgery.Then the degree of scar adhesion in each specimen was evaluated by Rydell method，the content of hydroxyproline（HOP），the area of peridural fibrosis formation and the count of fibroblasts were tested.Result：The latent period of SEP did not change obviously before and after administration in each group．The peridural adhesion，the content of HOP，the area of peridural fibrosis formation and the count of fibroblasts in group D，E decreased significantly compared with group A，B and C（P<0.05）.The peridural adhesion，the content of HOP and the count of fibroblasts in group B，C decreased significantly compared with group A（P<0.05），and no statistical difference with respect to the area of peridural fibrosis was noted（P＞0.05）.There was no statistical difference between group B and C，or between group D and E（P＞0.05）．Conclusion：0.5mg/ml MMC has the best dose-effect relationship and safety in preventing postlaminectomy peridural adhesion in rats．