Effect of bone mesenchymal stem cells transplantation on adrenocorticotropic hormone and corticosterone in rats with spinal cord injury

English Keywords:Spinal cord injury  Bone mesenchymal stem cells  Stress  Adrenocorticotropic hormone  Corticosterone

English Abstract:

  【Abstract】 Objective：To investigate the effect of bone mesenchymal stem cells（BMSCs） transplantation on adrenocorticotropic hormone and corticosterone in rats with spinal cord injury（SCI），and explore the potential anti-chronic stress mechanism.Method：A total of 48 adult male SD rats were divided into 3 groups equally and randomly：control group，SCI group and intervention group.Each group was divided into 2 subgroups based on time-point（21d and 35d after initiation）.Partial lower thoracic SCI was induced in SCI and intervention group by modified Allen′s method at T10.Rats in control group received laminectomy alone.At 7th day after SCI，the intervertebral disc of L4/5 was exposed；100μl of Hank′s buffered saline solution contained 1.0×106 rat BMSCs or the same amount of Hank′s buffered saline solution was injected into the subarachnoid space.The hind limb motor function was evaluated by Basso，Beattie and Bresnahan（BBB） scale and all animals′body weight pre-injection and at day 7，21，35 after operation were recorded.Rats in every subgroup were anesthetized at day 21，35 respectively，the adrenocorticotropic hormone（ACTH） and serum corticosterone（CORT） content by enzyme linked immunosorbent assay（ELISA） were tested respectively.Result：Before model production，in every group and at day 7，21，35 post-model production in control group，BBB scale were 21；at day 7 post-model production，there was no significant difference in BBB scale between injury and control group；at day 21 and 35 post-model production，BBB scale in treatment group were higher than those in injury group（P<0.05）.Both of treatment and injury group were lower than control gorup（P<0.05）.Before and 7 days after model production，there was no significant difference in animals′ body weight between the three groups；from day 21 post-model production，animals′ body weight in control group were higher than those in the injury and treatment group（P<0.05 and P<0.01） respectively；animals′ body weight in treatment group were higher than those in injury group（P>0.05）.At day 21 post-model production，the plasma ACTH content in model group were higher than control group（P<0.05）；but there was no significant difference in serum CORT content between the three groups.At day 35 post-model production，the plasma ACTH content in model group were higher than treatment group（P<0.05），and the serum CORT content in model group were higher than control group（P<0.05）.Conclusion：BMSCs transplantation could relieve chronic stress statement of SCI rats，which might achieve through regulation of ACTH and CROT secretion.