Comparison of culturing characteristics of rabbit intervertebral disc nucleus pulposus cells at different segments

English Keywords:Nucleus pulposus cells  Different segment  Cell viability  Cell culturing

English Abstract:

  【Abstract】 Objective：To investigate the culturing characteristics of nucleus pulposus cells from the neck、thoracic and lumbar intervertebral disc.Method：After anesthesia，the whole spine specimen was immediately separated from 10 white rabbits（3-4months） under the sterile conditions.Nucleus pulposus cells of different segments（C1/C2-C7/T1，T1/T2-T7/L1，L1/L2-L7/S1） were cultured and the attachment rates were recorded after 24h respectively.The proteoglycan contents was detected and the cell viability was accounted on 3，7，14，21 days.Result：There were statistical significances among every group with regard to attachment rate （P＜0.01）and the lumbar disc nucleus pulposus cells had the highest attachment rate.The difference of both the cell death/viability and proteoglycan contents among different segments on 3，7，14，21 days after culturing was significant（P＜0.01），and lumbar disc nucleus pulposus cells had the lowest death/viability rate（P＜0.01）and the highest proteoglycan contents（P＜0.01）.Conclusion：Lumbar disc nucleus pulposus cells，compared with the cervical and thoracic have a better growth status，which as a seed cell is more suitable for culturing.