The effects of AAV-hBMP-2 transfection to the rabbit degenerated intervertebal disces in vivo on the nucleus pulposus of apoptosis

English Keywords:Intervertebral disc degeneration  Apoptosis  Fas  Caspase-3  Rabbit

English Abstract:

  【Abstrast】 Objective：To study the effect of rabbit degenerated intervertebral disc pulpiform nucleus cells transfected with adeo-associated virus mediated ｈｕｍａｎ　bone morphogenetic protein-2（AAV-hBMP-2） in vivo on apoptosis of Fas and Caspase-3 in the nucleus pulposus.Method：A total of 36 New Zealand white rabbits in ordinary grade were established a rabbit model of intervertebral discs degeneration in L2/3、L3/4、L4/5 induced by puncturing the anulus fibrosus with defined needle.At four weeks postsurgery，the nucleus signal is lower than the normal in magnetic resonance imaging that proved the model of intervertebral disc degeneration to be successful.Then the rabbits of intervedebral disc degeneration were divided into three groups in random，named A，B and C.Then the groups A，B，C were injected AAV-hBMP-2（20μl，the virus titer is 6×106 pfu），AAV（20μl，the virus titer is 6×106 pfu） and normal saline（20μl） respectively.The analyses of radiology，TUNEL，immunohistochemistry with SP methods and IPP6.0 system were performed to examine the average optical of the apoptotic disc cells at two，four and eight weeks respectively after injection.Result：In the magnetic resonance imaging，within the group A，a remarkably highest signal intensity in T2-weighted images of the nucleus pulposus were detected in the three groups（P<0.05）.The group A of the average optical of the expression of Fas and Caspase-3 and the apoptotic cells were significantly lowest in the three groups（P<0.01），while there were no differences between the group B and C（P>0.05）.Conclusion：The AAV-hBＭＰ-2 transfection to the rabbit degenerated intervertebal disces in vivo could suppress apoptosis of degenerated intervertebal disces.