WANG Feng,WU Qijun,WANG Yuntao.Isolation and culturing of human degenerative intervertebral nucleus pulposus cells by solo collagenase Ⅱ and its morphology study[J].Chinese Journal of Spine and Spinal Cord,2010,20(4):300-304.
Isolation and culturing of human degenerative intervertebral nucleus pulposus cells by solo collagenase Ⅱ and its morphology study
【Abstract】 Objective:To investigate the feasibility of isolating and culturing of nucleus pulposus(NP) cells from degenerated human intervertebral discs by solo collagenase in vitro.Method:Nucleus pulposus from 20 human degenerated intervertebral disc sam