WANG Feng,WU Qijun,WANG Yuntao.Isolation and culturing of human degenerative intervertebral nucleus pulposus cells by solo collagenase Ⅱ and its morphology study[J].Chinese Journal of Spine and Spinal Cord,2010,20(4):300-304.
Isolation and culturing of human degenerative intervertebral nucleus pulposus cells by solo collagenase Ⅱ and its morphology study
Received:November 27, 2009  Revised:February 03, 2010
English Keywords:Intervertebral disc degeneration  Nucleus pulpusus cell  Collagenase  Cell culturing  Toluidine blue  Immunocytochemistry
Fund:江苏省自然科学基金资助项目(BK2007107);东南大学国家自然科学基金预研项目(XJ2008342)
Author NameAffiliation
WANG Feng Department of OrthopaedicsAffiliated Zhongda Hospital of Southeast UniversityNanjing Jiangsu210009China 
WU Qijun  
WANG Yuntao  
邵建树  
张 明  
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English Abstract:
  【Abstract】 Objective:To investigate the feasibility of isolating and culturing of nucleus pulposus(NP) cells from degenerated human intervertebral discs by solo collagenase in vitro.Method:Nucleus pulposus from 20 human degenerated intervertebral disc sam
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