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WANG Feng,WU Qijun,WANG Yuntao.Isolation and culturing of human degenerative intervertebral nucleus pulposus cells by solo collagenase Ⅱ and its morphology study[J].Chinese Journal of Spine and Spinal Cord,2010,20(4):300-304.

Isolation and culturing of human degenerative intervertebral nucleus pulposus cells by solo collagenase Ⅱ and its morphology study

Received:November 27, 2009 Revised:February 03, 2010

English Keywords:Intervertebral disc degeneration Nucleus pulpusus cell Collagenase Cell culturing Toluidine blue Immunocytochemistry

Fund:江苏省自然科学基金资助项目（BK2007107）；东南大学国家自然科学基金预研项目（XJ2008342）

Author Name	Affiliation
WANG Feng	Department of Orthopaedics，Affiliated Zhongda Hospital of Southeast University，Nanjing Jiangsu，210009，China
WU Qijun
WANG Yuntao
邵建树
张　明

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English Abstract:

【Abstract】 Objective：To investigate the feasibility of isolating and culturing of nucleus pulposus（NP） cells from degenerated human intervertebral discs by solo collagenase in vitro.Method：Nucleus pulposus from 20 human degenerated intervertebral disc sam

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