紧皮小鼠脊柱侧凸进展情况及其相关因素

The development of scoliosis and its associated factors in tight skin mouse

中文关键词:  脊柱侧凸  Micro-CT  紧皮小鼠  相关因素

英文关键词:Scoliosis  Micro-CT  Tight skin mouse  Risk factors

中文摘要:

  【摘要】 目的：观察紧皮小鼠（TSK鼠）脊柱侧凸进展情况及其相关因素，为临床预测脊柱侧凸进展情况提供参考。方法：36只2周龄TSK鼠按处死时间不同随机分为6组，每组6只，正常条件下饲养。饲养过程中在4、6、8、10、12和14周龄时称量小鼠体重并记录。分别在4、6、8、10、12和14周龄处死，提取脊柱骨骼标本并妥善保存。利用显微CT（micro-CT）对脊柱标本进行扫描，获得二维断层图像后导入Image J进行格式转换，用Mimics软件（14.11）三维建模，测量脊柱冠状位Cobb角和相关参数（脊柱后凸角度、全部13根肋骨的长度、全部6个腰椎的椎体宽度和高度）。利用Pearson相关性分析分别计算脊柱冠状位Cobb角与脊柱后凸角度、双侧肋骨长度差值、腰椎椎体宽度、腰椎椎体前缘高度、腰椎椎体后缘高度、腰椎椎体前后缘高度差值和体重的相关性。结果：TSK鼠4、6、8、10、12和14周龄时的脊柱冠状位Cobb角分别为7.69°±4.57°（3.26°~14.92°）、14.20°±7.52°（2.11°~21.66°）、11.50°±4.40°（4.78°~18.54°）、14.40°±6.23°（4.54°~22.12°）、17.38°±10.93°（4.12°~29.24°）和21.71°±7.73°（13.07°~33.88°）。脊柱冠状位Cobb角与脊柱后凸角度、双侧肋骨长度差值、腰椎椎体宽度、腰椎椎体前缘高度、腰椎椎体后缘高度、腰椎椎体前后缘高度差值和体重的相关系数分别为-0.053（P=0.769）、-0.138（P=0.444）、-0.186（P=0.301）、0.567（P=0.001）、0.554（P=0.001）、0.086（P=0.643）和0.482（P=0.005），脊柱冠状位Cobb角与腰椎椎体前缘高度、后缘高度和体重有相关性，与脊柱后凸角度、双侧肋骨长度差值、腰椎椎体宽度和腰椎椎体前、后缘高度差值无相关性。结论：TSK鼠脊柱冠状位Cobb角随年龄增大逐渐增加。TSK鼠脊柱侧凸进展情况与腰椎椎体前缘高度、后缘高度和体重密切相关，而与脊柱后凸角度、双侧肋骨长度差值、腰椎椎体宽度和腰椎椎体前、后缘高度差值无相关性。

英文摘要:

  【Abstract】 Objectives: To study the development of scoliosis and its risk factors in tight skin(TSK) mouse, and to evaluate the progression of scoliosis in clinical practice. Methods: 36 two-week-old TSK mice were divided into 6 groups(6 mice in each group) according to different time of sacrifice. The TSK mice were weighted at different time points(4 weeks, 6 weeks, 8 weeks, 10 weeks, 12 weeks and 14 weeks). Then the mice were euthanized on different time points(4 weeks, 6 weeks, 8 weeks, 10 weeks, 12 weeks and 14 weeks), and spine samples were collected and stored properly. Micro-computed tomography was used to scan all the spine samples to obtain cross sectional images, which were modified originally by image processing software "Image J". Then the processed images were imported into the software "Mimics 14.11" for 3D reconstruction and coronal Cobb angle and associated parameters,including kyphosis angle, rib length, vertebral width and height. Pearson correlation analysis was used to study the correlations of Cobb angle with kyphosis angle, rib difference between two sides, vertebral width, anterior vertebral height, posterior vertebral height, difference between anterior and posterior vertebral height, body weight. Results: The coronal Cobb angle was 7.69°±4.57°(3.26°-14.92°), 14.20°±7.52°(2.11°-21.66°), 11.50°±4.40°(4.78°-18.54°), 14.40°±6.23°(4.54°-22.12°), 17.38°±10.93°(4.12°-29.24°) and 21.71°±7.73°(13.07°-33.88°) on 4 weeks, 6 weeks, 8 weeks, 10 weeks, 12 weeks and 14 weeks old respectively. The correlation of Cobb angle with kyphosis angle, rib difference between two sides, vertebral width, anterior vertebral height, posterior vertebral height, difference between anterior and posterior height, weight was -0.053(P=0.769), -0.138(P=0.444), -0.186(P=0.301), 0.567(P=0.001), 0.554(P=0.001), 0.086(P=0.643) and 0.482(P=0.005) respectively. Conclusions: The coronal Cobb angle of TSK mice increases with their growth. The progress of scoliosis is correlated with anterior vertebral height, posterior vertebral height and weight in TSK mouse, while no with other parameters involved in our study.