双足直立诱导低褪黑素水平雌性小鼠脊柱侧凸模型的观察

The effect of bipedal amputation on a scoliosis model of female mouse with low melatonin level

中文关键词:  脊柱侧凸  低褪黑素水平  双足直立雌鼠

英文关键词:Scoliosis  Low melatonin level  Bipedal amputated female mice

中文摘要:

  【摘要】　目的：观察双足直立诱导低褪黑素水平雌性C57BL/6j小鼠脊柱侧凸的发生情况，为选择理想的特发性脊柱侧凸模型提供依据。方法：选取低褪黑素水平C57BL/6j小鼠雌、雄各20只，分为低褪黑素雌性小鼠组（A组）、低褪黑素雄性小鼠组（B组），另选C3He/ej雄性小鼠20只作为对照组（C组）。将三组小鼠均于３周龄时施行双上肢截肢合并鼠尾切除术建立双足直立小鼠模型，然后进行直立体态诱导。术后4个月，采用X线片评估三组小鼠脊柱侧凸发生情况，比较三组间脊柱侧凸发生率和侧凸严重程度。结果：三组小鼠建模后经直立体态诱导均可长时间保持直立体态。A组19只发生脊柱侧凸，侧凸小鼠Cobb角17°~36°，平均24.6°±6.0°；B组16只发生脊柱侧凸，侧凸小鼠Cobb角13°~31°，平均20.8°±4.3°；C组9只发生脊柱侧凸，侧凸小鼠Cobb角13°~23°，平均18.3°±2.7°。A、B组小鼠脊柱侧凸发生率均明显高于C组（χ2=11.904，P=0.005；χ2=5.2267，P=0.023），A、B两组脊柱侧凸发生率无显著性差异（P<0.05）。A组脊柱侧凸Cobb角明显高于B组（t=2.096，P=0.044）和C组（t=3.646，P=0.001）；B组与C组Cobb角无显著性差异（P>0.05）。结论：雌性低褪黑素双足直立小鼠脊柱侧凸发生率高，脊柱侧凸更严重，可以较好模拟特发性脊柱侧凸的发生发展过程，为特发性脊柱侧凸相关研究提供良好平台。

英文摘要:

  【Abstract】 Objectives: To establish a better adolescent idiopathic scoliosis(AIS) model of bipedal amputated mouse with low melatonin level. Methods: Twenty C57BL/6j female mice and twenty male mice were chosen to serve as low melatonin and divided into two groups according to gender, and twenty C3He/ej male mice were treated as controls. Amputation of forelimbs and tail was performed at 3 weeks old in three groups. After amputation, all these bipedal amputated mice were trained to keep a standing posture. Then X-rays were obtained at the 4th month to determine the development of spinal deformity. Comparisons on the incidence of scoliosis, and curve magnitude were performed among groups. Results: After establishing models, all mice were capable of keeping a standing posture for a long time. Scoliosis was observed in 19 out of 20 C57BL/6j female mice with an average Cobb angle of 24.6°±6.0°(range, 17° to 36°), in 16 out of 20 C57BL/6j male mice with an average Cobb angle of 20.8°±4.3°(range, 13° to 31°) and in 9 out of 20 controls with an average Cobb angle of 18.3°±2.7°(range, 13° to 23°). Conclusions: A higher scoliosis incidence and more severe curve are observed in C57BL/6j female bipedal mice compared with C57BL/6j male bipedal mice and control mice, which indicates that this model can be used for the research of AIS.