MMP-3、IgG和CD68在青少年与中老年突出腰椎间盘组织中的表达分析

MMP-3, IgG and CD68 expressions in the herniated nuclea pulposus between young and elder patients

中文关键词:  腰椎间盘突出  MMP-3  IgG  CD68  青少年  中老年

英文关键词:Lumbar intervertebral disc herniation  MMP-3  IgG  CD68  Adolescent  Elderly

中文摘要:

  【摘要】 目的：比较细胞因子基质金属蛋白酶3（matrix metalloproteinases 3，MMP-3）、IgG和CD68在青少年与中老年患者突出腰椎间盘组织中的表达，探讨两个年龄段腰椎间盘突出的病因。方法：收集40例腰椎间盘突出症患者的突出腰椎间盘髓核标本，青少年组18例，年龄11～25岁，平均20.6±3.4岁；中老年组22例，年龄40～72岁，平均48.0±10.3岁。HE染色观察标本退变情况，免疫组化染色检测MMP-3、IgG和CD68的表达，光学显微镜下观测并计录数据。结果：标本HE染色提示多数（17/22）中老年组腰椎间盘存在明显退变，青少年组多数椎间盘（13/18）退变不明显或无退变。MMP-3阳性率青少年组（33.33%）低于中老年组（81.82%）；IgG阳性率青少年组（66.67%）高于中老年组（31.82%）；CD-68阳性率青少年组（83.33%）高于中老年组（45.45%），两组MMP-3、IgG和CD68的表达阳性率比较，差异均有统计学意义（P<0.05）。结论：免疫和炎症反应可能是青少年腰椎间盘突出的重要病因，而中老年腰椎间盘突出可能主要与退变有关。

英文摘要:

  【Abstract】 Objectives: To compare the expressions of cytokines MMP-3(matrix metalloproteinases, MMPs), IgG and CD68 in the herniated nuclea pulposus between young and elder patients, and to explore the pathogenesis of lumbar intervertebral disc herniation in the both aged patients. Methods: Disc samples from 40 patients with lumbar intervertebral disc herniation were collected. The patients were grouped as the young group, including 18 patients, with an avereage age of 20.6±3.4 years; the elder group, 22 patients, with the avereage age of 48.0±10.3 years. Disc degeneration was detected by HE staing. The expressions of MMP-3, IgG and CD68 was detected by immunohistochemistry. Then data were collected and measured by optical microscope. Results: Discs in the elder group showed degeneration by HE staing, the opposite for the young group, even no degenerative change was noted. There were less expressions of MMP-3 in young group(33.33%) than elder group(81.82%), more expressions of IgG in young group(66.67%) than elder group(31.82%), and more expressions of CD68 in young group(83.33%) than elder group(45.45%). Positive expressions of MMP-3, IgG and CD68 in two groups were different. Conclusions: Immunative and inflammative reaction maybe the main mechanism inducing disc herniation in the young, while in the elderly, disc degeneration maybe the main cause.