腰椎间盘突出症与Th细胞、NK细胞的相关性研究

Correlation of lumbar disc herniation with the Th17 cells, NK cells

中文关键词:  腰椎间盘突出症  Th17细胞  NK细胞  细胞因子  白介素-17  流式细胞术

英文关键词:Lumbar disc herniation  Th17 cells  NK cells, Cytokine  IL-17  Flow cytometry

中文摘要:

  【摘要】 目的：探讨辅助性T细胞17（T help cell 17，Th17）、自然杀伤细胞（natural killer cell，NK）在腰椎间盘突出症发生发展中的作用，以求进一步明确神经根痛的病理机制。方法：选取南通大学附属医院脊柱外科收治接受手术治疗的腰椎间盘突出症患者20例作为观察组（椎间盘突出组），同时选取腰椎骨折需摘除椎间盘的患者6例作为对照组（正常髓核组）。运用流式细胞术（Flow Cytometry，FCM），分别测定观察组和对照组患者髓核组织中CD3、CD4、T细胞胞内产物白介素－17（IL－17）及NK细胞表面因子CD16CD56的含量。结果：观察组髓核中的CD4、IL-17和CD16CD56含量分别为（3.18±0.15）%、（2.94±0.04）%、（3.24±1.65）%，有高表达；而对照组髓核中的CD4、IL-17和CD16CD56含量分别为（0.08±0.02）%、0%、（1.73±0.71）%，低表达或者不表达，两组间比较差异有显著性（P值分别为0.000，0.000，0.003）。观察组与对照组髓核中CD3的表达分别为（23.42±5.84）％和（26.54±4.17）％，两组间差异无显著性（P＞0.05）。结论：髓核作为自身抗原可促使Th细胞分化成以IL-17为主的Th17细胞，并诱导CD16CD56为表面标志的NK细胞的表达，可能参与了腰椎间盘突出症的发生发展。

英文摘要:

  【Abstract】 Objectives: To explore the role of T help cells 17(Th17), natural killer cells(NK) in the development of sciatica and the pathological mechanism of nerve root pain. Methods: 20 postoperative patients with lumbar disc herniation and treated in the Spine Centre of Nan Tong University Affiliated Hospital were included as observation group. Control group included 6 patients with lumbar fracture in the same hospital. Flow cytometry (Flow Cytometry, FCM) was used to measure the expression of CD3, CD4, T cell intracellular product Th17 and NK cell surface factor CD16CD56 content in each group. Results: The CD4, IL-17 and CD16CD56 content in nucleus pulposus of observation group was (3.18±0.15)%, (2.94±0.04)%, (3.24±1.65)% respectively with hi expression; while in control group, it was (0.08±0.02)%, 0%, (1.73±0.71)% respectively, with lower expression or no expression(P=0.000, 0.000, 0.003). The expression of CD3 in observation group and control group was (23.42±5.84)% and (26.54±4.17)%, the difference was no statistically significant(P＞0.05). Conclusions: When exposed in the immune system, the Th cells on the surface of the nucleus pulpous may differentiate into Th17 cells, and induce the expression of NK cells with surface mark of CD16CD56.