Id-1，VEGF及微血管密度与脊柱转移瘤术中出血量及肿瘤来源间的关系

The relationship between Id-1, VEGF as well as microvessel density and blood loss in spinal metasta?鄄sis surgery and tumor origin

中文关键词:  脊柱转移瘤  Id-1  VEGF  微血管密度  免疫组化

英文关键词:Spinal metastasis  Id-1  VEGF  Microvessel density  Immunohistochemistry

中文摘要:

  【摘要】 目的：观察不同来源的脊柱转移肿瘤组织内Id-1、血管内皮生长因子（VEGF）及微血管密度（MVD）分布情况，探讨其与肿瘤来源及临床术中出血量的关系。方法：选取河北医科大学第四医院病理科2005年1月～2008年7月手术切除并经病理证实为脊柱转移瘤的石蜡包埋标本32例，术中出血量以病例实际记录为准，分为低、中、高出血量组，依据脊柱转移瘤来源分为上皮来源组和间叶组织来源组。采用免疫组化方法检测Id-1、VEGF和CD34（微血管标记）在32例脊柱转移瘤组织中的表达，分析各指标与手术术中出血量及肿瘤来源的关系。结果：Id-1与肿瘤来源有明显相关性，并与临床术中出血量呈正相关（P＜0.05）；不同来源的转移瘤中VEGF表达量不同，并与临床术中出血量呈正相关（P<0.05）；MVD在不同来源的转移瘤中比较没有统计学意义（P＞0.05）。不同来源的脊椎转移瘤术中出血量比较，有统计学意义（P<0.05）。结论：Id-1、VEGF在不同来源的脊柱转移瘤中的表达量具有明显区别，且与术中出血量呈正相关；上皮来源的脊柱转移瘤术中出血量大于间叶来源组。不同来源的脊柱转移瘤中，微血管密度无明显不同。

英文摘要:

  【Abstract】 Objectives: To investigate the distributions of Id-1, vascular endothelial growth factor (VEGF)　and microvessel density (MVD) in metastatic spinal tumor with different origins and their association with intraoperative blood loss. Methods: 32 metastases spinal specimens confirmed by pathological method in our hospital from 2005 to 2008 were collected. Based on the amount of blood loss, all cases were divided into low, medium and high blood loss subgroup, and all specimens were divided into epithelial group and mesenchymal derived group based on the origins of metastases. Immunohistochemical staining was used to detect Id-1, VEGF and CD34(microvascular markers)　in all specimens. The above-mentioned index, tumor origin and blood loss were analyzed each other. Results: Id-1 associated significantly with tumor origin(P<0.05), and showed positive relationship with intraoperative blood loss; Metastases from different origins had different expressions of VEGF (P<0.05), which were positively correlated with the amount of blood loss; no significant difference was noted between MVD and metastases with different origins (P＞0.05). Spinal metastases with different origins showed statistical significance　with blood loss(P<0.05). Conclusions: Id-1 and VEGF expressions are different as for different metastasis origins, and are positively correlated with blood loss. Microvessel density is not significantly influenced by origin of spinal metastases. The epithelial origin metastases are associated with more blood loss.