陈宣维,林文平,林建华.慢病毒介导的脑红蛋白体内基因转染对兔损伤脊髓的作用[J].中国脊柱脊髓杂志,2012,(3):272-277. |
慢病毒介导的脑红蛋白体内基因转染对兔损伤脊髓的作用 |
中文关键词: 脊髓损伤 脑红蛋白 转基因 丙二醛 一氧化氮 |
中文摘要: |
【摘要】 目的:观察慢病毒介导脑红蛋白(Ngb)体内基因转染兔损伤的脊髓组织后对后肢运动功能的影响,探讨其作用机制。方法:用球囊压迫法制成兔脊髓损伤(SCI)模型96只,随机分为对照组(A组)、生理盐水组(B组)、空载体组(C组)和Ngb慢病毒组(D组),每组动物24只,A组SCI后无治疗;B组SCI后向脊髓内注射生理盐水;C组SCI后向脊髓内注射空病毒;D组SCI后向脊髓内注射Ngb重组慢病毒。各组分别在1、3、7、14、21d采用BBB运动功能评分系统检测兔后肢运动功能情况;观察损伤脊髓组织内标记荧光的表达;Real-time PCR和Western blot检测Ngb mRNA及其相应蛋白的表达情况,生化方法检测丙二醛(MDA)和一氧化氮(NO)水平。结果:损伤后14d和21d,D组BBB评分明显高于其他3组(P<0.05),但A、B及C组之间比较无差异(P>0.05);C组和D组兔损伤部位脊髓组织均有GFP表达的绿色荧光信号;损伤后7d、14d和21d,D组的Ngb表达与其他3组比较明显增强(P<0.05);损伤后7d、14d和21d,D组损伤脊髓组织中MDA、NO含量明显低于其他3组(P<0.05)。结论:慢病毒介导脑红蛋白(Ngb)体内基因转染可使Ngb高表达,可能是通过减轻继发性SCI,从而促进SCI后后肢运动功能的恢复。 |
The protection of lentivirus-mediated neuroglobin gene transfer in vivo on injured spinal cord in rabbits, an experimental study |
英文关键词:Spinal cord injury Neuroglobin Gene transfer Malondialdehyde Nitric oxide |
英文摘要: |
【Abstract】 Objectives: To investigate the possible protective mechanism of lentivirus-mediated neuroglobin gene transfer in vivo on injured spinal cord (SCI) in rabbits. Methods: Ninety-six New Zealand white rabbits were randomly divided into four groups(n=24) according to intervention after SCI: no intervention(A); normal saline group(B); LV-GFP group (C); and LV-Ngb group(D).SCI was introduced by epidural balloon compression.24 hours after compression, T9 laminectomy was performed, then normal saline, LV-GFP or LV-Ngb was injected in situ respectively.Motor function of hind limbs after injection was evaluated by Basso-Beattie-Bresnahan(BBB) score, the expressions of green fluorescence in injured spinal cord were observed by fluorescence microscope, the expressions of Ngb mRNA and protein in injured spinal cord were detected by real-time PCR and Western blot, and the levels of malondialdehyde (MDA) and nitric oxide (NO) were also tested. Results: Significant functional improvement was observed in group D compared with group A, B and C at day 14 and 21.The expressions of green fluorescence in injured spinal cord were observed in group C and D.The Ngb expression in group D increased significantly compared with the other 3 groups at day 7, 14 and 21.The MDA and NO levels in group D were lower than the other 3 groups(P<0.05) at day 7, 14 and 21. Conclsions: Lentivirus-mediated neuroglobin gene transfer in vivo may induce high expression of Ngb, which may be resulted from decreasing secondary spinal cord injury, thus promoting hind limbs motorfunction. |
投稿时间:2011-07-31 修订日期:2011-10-31 |
DOI:10.3969/j.issn.1004-406X.2012.3.272.5 |
基金项目: |
|
摘要点击次数: 3433 |
全文下载次数: 2620 |
查看全文 查看/发表评论 下载PDF阅读器 |
关闭 |