大鼠急性颈脊髓损伤后P物质与肺水肿的关系

Role of substance P in developing pulmonary edema after acute cervical spinal cord injury in rats

中文关键词:  脊髓损伤  肺水肿  P物质  大鼠

英文关键词:Spinal cord injury  Pulmonary edema  Substance P  Rat

中文摘要:

  【摘要】 目的：探讨大鼠急性颈脊髓损伤后P物质与肺水肿的关系。方法：成年Wistar大鼠40只，体重240~250g，雌雄不限，随机分为实验组和对照组，每组20只，每组又分为造模后24h、3d、1周、2周共4个时间点，每个时间点5只大鼠。实验组采用改良Allen′s打击法制作大鼠C7急性脊髓损伤（SCI）模型，打击力度为10×2.5g·cm；对照组只暴露C7段脊髓。在造模后相应时间点处死大鼠，通过光学显微镜观察肺组织的病理学变化，并检测各时间点两组大鼠血清和肺泡灌洗液中P物质含量和蛋白浓度，计算肺通透指数（肺泡灌洗液蛋白浓度/血清蛋白浓度）。结果：实验组大鼠急性SCI后24h肺组织可见肺毛细血管充血，肺泡内分泌物增多、红细胞增加；伤后3d达到高峰；伤后1周肺泡内水肿、出血较3d时减轻；伤后2周明显好转；对照组肺泡透亮，无水肿和出血。实验组伤后24h血清和肺泡灌洗液中P物质升高，伤后3d达高峰，伤后1周开始下降，伤后2周明显下降；实验组伤后24h、3d、1周血清及伤后24h、3d肺泡灌洗液中P物质含量与对照组比较有显著性差异（P<0.05），伤后2周两组比较无显著性差异（P>0.05）。肺通透指数的变化与P物质的变化趋势相同。结论：大鼠急性颈SCI后肺泡灌洗液及血清中P物质含量变化与肺水肿程度变化同步，P物质可能参与了急性颈SCI后肺水肿的形成过程。

英文摘要:

  【Abstract】 Objective：To investigate the relationship between pulmonary edema and substance P in rats suffering ｆｒｏｍ　acute cervical spinal cord injury.Method：40 Wistar rats with 240-250g in weight，were randomly divided into two groups：control group（n=20） and injury group（n=20），and the latter ｗａｓ subdivided into groups of 24h，3d，1 week，2 weeks after injury respectively.The spinal cord injury（SCI） models were induced at the level of C7 vertebra by dropping a weight of 10g from the height of 25mm （Allen′s model）.While in control group，laminas were removed only，leaving spinal cord at the same level intact.All the animals were sacrificed at each time point ranging from 24h to 2 weeks（24h，3d，1 week，2 weeks）.Substance P and protein concentration in serum and bronchoalveolar lavage fluid（BALF） were measured.The lung permeability index（LPI） and change of the pulmonary pathology were calculated.Result：In injury group，lung slices showed pulmonary capillaries congestion at 24ｈ after injury，ａｎｄ　the pulmonary alveoli were filled with erythrocytes and serous extravagated，which peaked at 3d，and began ease at 1 week.The content of substance P in serum and BALF began increasing at 24h，reached peak at 3d，declined at 1 week，and declined obviously at 2 weekｓ.There was statistical significance with respect to the content of substance P in serum between control group and injury group at 24h，3d and 1 week（P<0.05），and the deviation of LPI was similar to that of substance P.Conclusion：The deviation of substance P in serum and BALF is consistent with the degree of pulmonary edema in acute cervical spinal cord injury in rats.Substance P may take part in the development of pulmonary edema in SCI rats.