骨髓间充质干细胞移植对脊髓损伤大鼠促肾上腺皮质激素和皮质酮的影响

Effect of bone mesenchymal stem cells transplantation on adrenocorticotropic hormone and corticosterone in rats with spinal cord injury

中文关键词:  脊髓损伤  骨髓间充质干细胞  应激  促肾上腺皮质激素  皮质酮  大鼠

英文关键词:Spinal cord injury  Bone mesenchymal stem cells  Stress  Adrenocorticotropic hormone  Corticosterone

中文摘要:

  【摘要】 目的：观察骨髓间充质干细胞（BMSCs）移植对脊髓损伤（SCI）大鼠促肾上腺皮质激素和皮质酮的影响，探讨BMSCs移植治疗SCI的潜在抗慢性应激机制。方法：成年雄性SD大鼠48只，随机分为对照组、损伤组和治疗组，每组16只，每组动物再根据取材时间点（造模后21d、35d）平均分为2个亚组。损伤组和治疗组采用改良Allen法建立大鼠T10 SCI模型；对照组仅显露硬膜囊，不损伤脊髓。造模后7d，对照组及治疗组于L4/5间隙蛛网膜下腔注射100μl含1.0×106 个BMSCs的Hank′s缓冲液混悬液，损伤组注射同体积Hank′s缓冲液。术前、造模后7d、21d、35d以BBB评分评估大鼠后肢运动功能，同时记录动物体重。根据分组分别于造模后21d、35d处死动物，取左心室血，以酶联免疫吸附测定法（ELISA）测定血浆促肾上腺皮质激素（ACTH）和血清皮质酮（CORT）含量。结果：造模前三组大鼠、造模后对照组各时间点BBB评分均为21分，造模后7d损伤组BBB评分与治疗组比较无显著性差异，造模后21d和35d治疗组BBB评分均高于损伤组（P<0.05），且两组均低于对照组（P<0.05）。造模前和造模后7d三组大鼠体重无显著性差异，造模后21d和35d，对照组大鼠体重大于损伤组和治疗组（P<0.05），治疗组与损伤组比较无显著性差异（P>0.05）。造模后21d，损伤组血浆ACTH含量高于对照组，差异有显著性（P<0.05）；治疗组与对照组无显著性差异（P>0.05）。损伤组与治疗组血清皮质酮均低于对照组，但无显著性差异（P>0.05）。造模后35d，损伤组和治疗组血浆ACTH含量均比21d时下降，治疗组下降有显著性意义（P<0.05），且治疗组明显低于损伤组（P<0.05）；损伤组和治疗组血清皮质酮含量均比21d时升高，损伤组升高有显著性意义（P<0.05），且明显高于对照组（P<0.05）。结论：BMSCs移植可改变SCI大鼠促肾上腺皮质激素和皮质酮的分泌，从而缓解其慢性应激状态。

英文摘要:

  【Abstract】 Objective：To investigate the effect of bone mesenchymal stem cells（BMSCs） transplantation on adrenocorticotropic hormone and corticosterone in rats with spinal cord injury（SCI），and explore the potential anti-chronic stress mechanism.Method：A total of 48 adult male SD rats were divided into 3 groups equally and randomly：control group，SCI group and intervention group.Each group was divided into 2 subgroups based on time-point（21d and 35d after initiation）.Partial lower thoracic SCI was induced in SCI and intervention group by modified Allen′s method at T10.Rats in control group received laminectomy alone.At 7th day after SCI，the intervertebral disc of L4/5 was exposed；100μl of Hank′s buffered saline solution contained 1.0×106 rat BMSCs or the same amount of Hank′s buffered saline solution was injected into the subarachnoid space.The hind limb motor function was evaluated by Basso，Beattie and Bresnahan（BBB） scale and all animals′body weight pre-injection and at day 7，21，35 after operation were recorded.Rats in every subgroup were anesthetized at day 21，35 respectively，the adrenocorticotropic hormone（ACTH） and serum corticosterone（CORT） content by enzyme linked immunosorbent assay（ELISA） were tested respectively.Result：Before model production，in every group and at day 7，21，35 post-model production in control group，BBB scale were 21；at day 7 post-model production，there was no significant difference in BBB scale between injury and control group；at day 21 and 35 post-model production，BBB scale in treatment group were higher than those in injury group（P<0.05）.Both of treatment and injury group were lower than control gorup（P<0.05）.Before and 7 days after model production，there was no significant difference in animals′ body weight between the three groups；from day 21 post-model production，animals′ body weight in control group were higher than those in the injury and treatment group（P<0.05 and P<0.01） respectively；animals′ body weight in treatment group were higher than those in injury group（P>0.05）.At day 21 post-model production，the plasma ACTH content in model group were higher than control group（P<0.05）；but there was no significant difference in serum CORT content between the three groups.At day 35 post-model production，the plasma ACTH content in model group were higher than treatment group（P<0.05），and the serum CORT content in model group were higher than control group（P<0.05）.Conclusion：BMSCs transplantation could relieve chronic stress statement of SCI rats，which might achieve through regulation of ACTH and CROT secretion.