阔筋膜修复腰椎间盘纤维环的组织学观察

Histological study of fascia lata on repairing intervertebral disc annulus

中文关键词:  腰椎间盘  阔筋膜  修复  纤维环  组织学  兔

英文关键词:Lumbar intervertebrae disc  Fascia lata  Annulu  Repairing  Histology  Rabbit

中文摘要:

  【摘要】 目的：探讨阔筋膜修复腰椎间盘纤维环的可行性。方法：16只健康成年新西兰大耳白兔，经腹膜外入路切开L4/5椎间盘纤维环，摘除髓核，模拟临床人工髓核置换术方法置入人工髓核，用自体阔筋膜修复纤维环切口。分别于术后4、8、12及16周处死动物，取手术节段椎间盘进行大体和组织形态学观察。结果：术后4周，阔筋膜表面有薄层肉芽组织增生，少量炎性细胞浸润；阔筋膜纤维中未见炎性细胞浸润。术后8周，阔筋膜表面有较多纤维肉芽组织形成和炎性细胞浸润；阔筋膜纤维中未见炎性细胞浸润。术后12周时，大量连贯的纤维肉芽组织封闭阔筋膜表面，其中可见大量炎性细胞；阔筋膜纤维中未见炎性细胞浸润。术后16周时，纤维肉芽组织、阔筋膜与纤维环之间紧密结合；阔筋膜纤维中未见炎性细胞浸润；阔筋膜维持原有的正常结构：胶原纤维纵向排列，其纤维细胞呈梭形，与纤维走行方向一致。结论：用兔自体阔筋膜修复腰椎间盘纤维环切口移植的阔筋膜不被受区组织吸收降解，能保持其原有的组织结构，从而保持它固有的坚韧力学性能。

英文摘要:

  【Abstract】 Objective：To investigate the feasibility of fascia lata on repairing intervertebral disc annulus.Method：16 healthy New Zealand rabbits were divided into 4 groups randomly.Intervertebral disc（L4/5） was exposed and removed by retroperitoneal approach in every rabbit.Then the artificial nucleus was implanted into disc cavity，and the incision of annulus was sewed by fascia lata.The animals were sacrificed at 4 weeks，8 weeks，12 weeks and 16 weeks after operation respectively，and the gross anatomical and histomorphology changes were studied.Result：All incisions of annulus were healed after operations.No protrusion of artificial nucleus was observed.4 weeks after operation，there was a thin layer of fibro granulation tissue developed on the surface of the fascia lata，and a few inflammatory cells were noted infiltrating into fascia lata.At 8 weeks，fibre granulation tissues developed much more than before，and more inflammatory cells could be observed， while no inflammatory cells were noted infiltrating into fascia lata.At 12 weeks，much more fibre granulation tissues blocked the surface of fascia lata.Inflammatory cells were evidenced and no inflammatory cells were noted infiltrating into fascia lata.At 16 weeks，fibre granulation tissues，fascia lata and annulus grew intersectionally，and no inflammatory cells were noted infiltrating into fascia lata，and fascia lata maintained the original structure with the fibra tissue orientated longitudinally and parallelly.Conclusion：Repairment of lumbar intervertebral disc annulu incision with fiscia lata is not apt to degradation，which can maintain its inherent structure as well as biomechanical properties.