大鼠脊髓损伤后Wnt信号分子的表达变化

The expression of Wnt signaling gene after spinal cord injury in rats

中文关键词:  脊髓损伤  Wnt-1  β-连锁蛋白  糖原合成酶激酶-3β

英文关键词:Spinal cord injury  Wnt-1  β-catenin  Glycogen synthase kinase-3β

中文摘要:

  【摘要】 目的：探讨大鼠脊髓损伤后不同时期Wnt信号分子Wnt-1、β-连锁蛋白（β-catenin）及糖原合成酶激酶-3β（GSK-3β）在脊髓损伤局部的表达情况。方法：50只成年雌性SD大鼠随机均分为对照组和实验组，麻醉下手术显露T9~T11椎板，切除T10全椎板，实验组大鼠用NYU打击器以10g×5cm的打击能量致伤T10脊髓，对照组只行全椎板切除，不致伤脊髓。分别于术后1d、3d、5d、7d、14d每组各取5只大鼠，取以损伤区为中心共15mm范围内（对照组取相应部位）脊髓组织，提取总RNA，采用半定量RT-PCR的方法检测脊髓组织中Wnt-1、β-catenin及GSK-3β的mRNA表达量。结果：脊髓损伤后1d和3d时Wnt-1和β-catenin出现高表达，5d后其表达逐渐减弱，14d左右其表达基本恢复到正常水平，而在脊髓损伤后1d和3d时GSK-3β呈低表达，5d后其表达逐渐增强，各时间点之间差异有显著性（P<0.05）。对照组中Wnt-1和β-catenin及GSK-3β均呈低表达，各时间点表达无显著差异（P>0.05）。结论：大鼠脊髓损伤后损伤局部脊髓组织中Wnt-1、β-catenin及GSK-3β的表达发生变化，提示Wnt信号在脊髓损伤后的早期被激活，其可能与脊髓损伤后的修复再生有关。

英文摘要:

  【Abstract】 Objective：To observe the changes of expression of Wnt signaling gene Wnt-1，β-catenin and glycogen synthase kinase-3β（GSK-3β） at different time period following spinal cord injury in rats.Method：Fifty adult female SD rats were randomly divided into two groups：the sham group（only laminectomy，n=25） and the spinal cord injury（SCI） group（n=25）.SCI model was established by hitting T10 spinal cord with NYU impactor in the force of 10g×5cm.The total RNA of the spinal cord tissue was extracted for the examination of the mRNA expression of Wnt-1，β-catenin and GSK-3β by using RT-PCR at the 1，3，5，7 and 14 days after spine injury or laminectomy.Result：In the SCI group，the expression of Wnt-1 and β-catenin mRNA had a notable increase at 1 and 3 days after injury，then the expression began to decrease at 5 days after injury and it was almost back to normal after 14 days.GSK-3β mRNA showed low expression at 1 and 3 days after injury and the expression began to increase at 5 days later.The lower expression of Wnt-1，β-catenin and GSK-3β was observed in the sham group and presented no significant difference in the expressions among the different time periods（P>0.05）.Conclusion：The expression changes of Wnt-1，β-catenin and GSK-3β　mRNA after spinal cord injury suggest that Wnt signaling may play an important role in the neural regeneration after spinal cord injury.