大鼠脊髓损伤致截瘫后肠道细菌移位的实验研究

Study of bacterial translocation from gut after paraplegia caused by spinal cord injury in rats

中文关键词:  大鼠 脊髓损伤 截瘫 肠道细菌移位 实验 并发症

英文关键词:Spinal cord injury,Paraplegia,Complication,Rat,

中文摘要:

  目的：探讨大鼠脊髓损伤致截瘫后是否发生肠道细菌移位。方法：建立大鼠脊髓损伤性截瘫模型，以脊髓损伤性截瘫后12h、24h、48h大白鼠为实验组，未损伤脊髓的正常大白鼠为对照组。在无菌条件下，采集动物下腔静脉血进行内毒素定量测定和细菌培养，采集肝、脾、肠系膜淋巴结、肠腔内容物作细菌培养并进行菌种鉴定。取实验组和对照组各动物的肝、脾、肠系膜淋巴结、空肠、回肠进行病理切片HE染色检查，取空、回肠进行电镜检查。结果：大鼠脊髓损伤致截瘫后24h开始出现内毒素血症，截瘫后48h出现细菌移位。结论：大鼠脊髓损伤致截瘫后将发生肠道细菌移位，提示脊髓损伤截瘫的病人应尽早给予抗生素治疗。

英文摘要:

  Objective:To investigate whether gut origin bacterial translocation(BT)occurs after spinal cord in-jury(SCI)with paraplegia in rats.Method:Model of paraplegia was built up by injuring spinal cord of rat.The experimental group was rats of paraplegia,the co