电针与低频脉冲电穴位刺激对新西兰兔尿潴留模型排尿效果影响的比较

Comparison of the effectiveness of electronic acupuncture and low frequency pulse to enhance the urination in a rabbit urinary retention model

中文关键词:  尿潴留  排尿效果  低频脉冲  电针  穴位刺激  兔

英文关键词:Urine retention  Urination  Low frequency pulse  Electric acupuncture  Rabbits

中文摘要:

  【摘要】 目的：比较电针与低频脉冲电穴位刺激对膀胱逼尿肌功能减弱型新西兰雄兔尿潴留模型排尿效果的影响。方法：选取3月龄新西兰雄兔40只，随机分为正常组、对照组、电针刺激组和低频脉电冲刺激组，每组10只。在全麻状态下，4组动物均经尿道插入尿管，用注射器抽吸空膀胱后，以50ml/h的速度向膀胱内灌注生理盐水，在灌注第50min时，对照组、电针刺激组以及低频脉冲电刺激组同时以20ml/h 的速度匀速静脉注射0.05%山莨菪碱10ml建立逼尿肌功能减弱型膀胱模型，正常组则等速静脉注射等量生理盐水。干预措施：当膀胱灌注完毕（第90min）时，取中极穴与一侧三阴交穴位进行刺激，时间20min。电针刺激组在穴位上针刺留针，并连接电针仪，用2Hz的频率、0.6A的电流持续刺激；低频脉冲电刺激组在穴位上贴电极片，连接TERESA神经肌体仪用20~100Hz的频率、100~500μs脉冲宽度刺激；对照组与正常组不采用任何刺激，休息20min。用生理信号采集系统仪器测量记录4组动物造模后即刻（干预措施前）时、干预措施结束时的膀胱内压力及干预措施结束后即刻从尿管排尿5min的平均排尿速度。结果：干预措施前，低频脉冲电刺激组（12.6±1.5cmH2O）、电针刺激组（13.5±1.6cmH2O）、对照组（12.5±1.5cmH2O）的膀胱内压力显著低于正常组（16.7±3.2cmH2O）（P＜0.05）；实施干预措施后，低频脉冲电刺激组（16.8±1.6cmH2O)、电针刺激组（17.1±1.7cmH2O）的膀胱内压力均明显高于对照组（11.6±1.4cmH2O）（P＜0.05），也均高于同组造模后即刻（干预措施前）时（P＜0.05）。低频脉冲电刺激组（106.2±10.2滴/分）、电针刺激组（102.3±10.5滴/分）、正常组（105.7±11.4滴/分）的平均排尿速度均显著高于对照组（84.4±9.7滴/分），差异均有统计学意义（P＜0.05）。低频脉冲电刺激组膀胱内压力与平均排尿速度与电针刺激组比较均无统计学差异（P＜0.05）。结论：电针与低频脉冲穴位刺激均能有效改善新西兰雄兔尿潴留模型尿潴留情况，促进排尿，但低频脉冲穴位刺激操作简便，避免了侵入性操作，安全性较好。

英文摘要:

  【Abstract】 Objectives: To compare the difference of the enhancing effect of electronic acupuncture and low frequency pulse acupoint stimulation on the urination potential in a rabbit urinary retention model. Methods: 40 male New Zealand rabbits were used and randomly divided into normal group(n=10), control group(n=10), electronic acupuncture group(EA group, n=10) and low frequency group(n=10). Under the condition of general anesthesia, four groups of animals were inserted transurethral tube, suction empty bladder with a syringe, at a speed of 50ml/h to the bladder perfusion physiological saline, in 50min reperfusion, control group EA group and low frequency group at the same time at a speed of 20ml/h uniform intravenous 0.05% mountain scopolamine 10ml model type weakening in function of forced urinous muscle, bladder, and normal group, constant intravenous injection amount of saline solution. Interventions: when the bladder perfusion(90min), taking in A hole on one side and three Yin meridians stimulated, time to 20min EA group on the acupuncture needle retaining needle, electric acupuncture apparatus and connections, 2Hz frequency current of 0.6A constant stimulation was used. The low frequency group attached electrodes to the acupuncture points, which were connected to the frequency of 20-100Hz of the TERESA nerve body instrument, which stimulated the width of the pulse width of 100 to 500μs. No stimulus was used in control group and normal group, the rest for 20min; physiological signal acquisition system instrument was used to measure four groups of animals when the bladder perfusion(before intervention) over at the end of the intervention, and the end of intervention measures immediately from the urine tube average urination urine speed(5min). Results: Intervention measures four groups of animals before the pressure on the bladder and low frequency group(12.6±1.5cmH2O), EA group(13.5±1.6cm H2O), in control group(12.5±1.5cmH2O) was significantly lower than normal group(16.7±3.2cmH2O). After intervention measures, low frequencygroup(16.8±1.6cmH2O) was significantly higher than control group(11.6±1.4cmH2O), which was also higher than the group with the end of bladder perfusion(before intervention). Micturition speed comparison showed low frequency group(106.2±10.2 drops/min), EA group(102.3±10.5 drops/min), normal group(105.7±11.4 drops/min) were significantly higher than control group(84.4±9.7 drops/min)(all P<0.05); in the low frequency group and EA group, the inside bladder pressure and average micturition speed showed no statistical significance(P＞0.05). Conclusions: Both the electric acupuncture and low frequency pulse acupoint stimulation are effective to improve the urination of urinary retention in rabbits; low frequency pulse acupoint stimulation is easily accessible without invasive operations which is more suitable for extensively clinical application.