不同剂量嗅鞘细胞蛛网膜下腔移植修复脊髓损伤的实验研究

Different doses of ｏlfactory ensheathing cells transplantation in subarachnoid for repairment of spinal cord injury, an experimental study

中文关键词:  嗅鞘细胞  脊髓损伤  蛛网膜下腔移植  移植剂量

英文关键词:Olfactory ensheathing cells  Spinal cord injury  Subarachnoid transplantation  Dose

中文摘要:

  【摘要】 目的：探讨嗅鞘细胞蛛网膜下腔移植治疗脊髓损伤的可行性、安全性及细胞移植数量与疗效的关系。方法：应用改良Allen′ｓ法制备大鼠T10脊髓损伤模型，随机分四组：大剂量嗅鞘细胞蛛网膜下腔移植组（A组，2×106个）、中剂量嗅鞘细胞蛛网膜下腔移植组（B组，2×105个）、小剂量嗅鞘细胞蛛网膜下腔移植组（C组，2×104个）、DMEM/F12培养液蛛网膜下腔移植组（D组）。术后即刻进行细胞移植，术后第４、１０周行CBS功能评分及组织学检查，观察脊髓神经功能修复情况及损伤区神经纤维数量。结果：CBS功能评分示A、B组明显好于C、D两组（P＜0.05）；A、B两组间比较无差异，C、D两组间比较无差异（P＞0.05）。组织学观察发现A、B组神经纤维数量多于C、D组。结论：急性期嗅鞘细胞经蛛网膜下腔移植治疗脊髓损伤安全有效，急性期移植细胞数量较大则脊髓神经功能恢复较好。

英文摘要:

  【Abstract】 Objective：To investigate the feasibility, safety and dose-effect relationship of olfactory ensheathing cell transplantation for spinal cord injury.Method：Rats with T10 spinal cord injury induced by modified Allen method were randomly divided into four groups and received immediate cell transplantation as followings：high-dose olfactory ensheathing cells（OECs）　transplantation in subarachnoid（group A），medium－dose transplantation（group B），low-dose transplantation（group C），control group with DMEM/F12 transplantation（group D）.The neurofunctional recovery was noted by combined behavioral scores（CBS）；ｔhe axons regeneration and the survival of olfactory ensheathing cells at the different time-point recorded either.Results：The neurofunction of group A and B improved significantly compared with group C or D according to combined behavioral scores（CBS），whereas no difference was noted between group A and B，as well as same as between group C and D.Ｉｔ　ｗａｓ　ｆｏｕｎｄ　ｂｙ　ｈｉｓｔｏｌｏｇｉｃａｌ　ｏｂｓｅｒｖａｔｉｏｎ　ｔｈａｔ the number of nerve fibers of group A and group B wａｓ more than group C and D.Conclusion：The OEC transplantation in subarachnoid for acute spinal cord injury is reliable, and high dose showｓ good prognosis.