兔不同节段椎间盘髓核细胞培养特性的比较

Comparison of culturing characteristics of rabbit intervertebral disc nucleus pulposus cells at different segments

中文关键词:  髓核细胞  不同节段  细胞活力  细胞培养

英文关键词:Nucleus pulposus cells  Different segment  Cell viability  Cell culturing

中文摘要:

  【摘要】 目的：探讨兔颈段、胸段及腰段椎间盘髓核细胞的培养特性。方法：3~4月龄兔10只，麻醉后，在无菌条件下手术分离整段脊柱，分别取颈段（C1/2~C7/T1）、胸段（T1/2~T7/L1）、腰段（L1/2~L7/S1）髓核细胞进行培养，于培养后的24h计算贴壁率。并于贴壁后的第3、7、14及21天计算细胞死亡／成活比，判断细胞活力并检测蛋白多糖的含量。结果：颈段、胸段、腰段细胞贴壁率差异具有显著性，腰段细胞贴壁率最高（P＜0.01）。不同节段之间，在多个时间点观察髓核细胞死亡／存活比差异具有显著性，腰段椎间盘髓核细胞的死亡／成活比最低（P＜0.01）。不同节段之间，不同时间点观察的蛋白多糖含量差异具有显著性，且腰段椎间盘髓核细胞的蛋白多糖含量最高（P＜0.01）。结论：腰段椎间盘髓核细胞较颈段及胸段的髓核细胞生长状态好，兔腰椎髓核细胞更适宜作为细胞培养的种子细胞。

英文摘要:

  【Abstract】 Objective：To investigate the culturing characteristics of nucleus pulposus cells from the neck、thoracic and lumbar intervertebral disc.Method：After anesthesia，the whole spine specimen was immediately separated from 10 white rabbits（3-4months） under the sterile conditions.Nucleus pulposus cells of different segments（C1/C2-C7/T1，T1/T2-T7/L1，L1/L2-L7/S1） were cultured and the attachment rates were recorded after 24h respectively.The proteoglycan contents was detected and the cell viability was accounted on 3，7，14，21 days.Result：There were statistical significances among every group with regard to attachment rate （P＜0.01）and the lumbar disc nucleus pulposus cells had the highest attachment rate.The difference of both the cell death/viability and proteoglycan contents among different segments on 3，7，14，21 days after culturing was significant（P＜0.01），and lumbar disc nucleus pulposus cells had the lowest death/viability rate（P＜0.01）and the highest proteoglycan contents（P＜0.01）.Conclusion：Lumbar disc nucleus pulposus cells，compared with the cervical and thoracic have a better growth status，which as a seed cell is more suitable for culturing.