杜劲松,王剑,张信英.诱导成鼠骨髓源神经干细胞的实验研究[J].中国脊柱脊髓杂志,2004,(8):-485,i001.
诱导成鼠骨髓源神经干细胞的实验研究
Study of induction of neural stem cells derived from adult rat bone marrow
  
DOI:
中文关键词:  诱导 鼠 骨髓源 神经干细胞 实验研究
英文关键词:Bone marrow,Neural stem cell,Differentiation,
基金项目:
杜劲松  王剑  张信英
哈尔滨医科大学附属第一医院骨科 150001黑龙江省哈尔滨市 (杜劲松,王剑)
,哈尔滨医科大学附属第一医院骨科 150001黑龙江省哈尔滨市(张信英)
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中文摘要:
  目的:探讨体外诱导成鼠骨髓源神经干细胞的可行性。方法:以含20ng/ml碱性成纤维生长因子(basic fibroblast growth factor,bFGF)与表皮生长因子(epidermal growth factor,EGF)的无血清培养液培养成鼠骨髓细胞,待细胞球形成后进行传代培养与单细胞克隆试验.以含100ng/ml bFGF培养液诱导分化,细胞免疫组化染色进行细胞鉴定。结果:骨髓细胞在诱导条件下形成表达Nestin抗原的细胞球.亦表达造血干细胞标志性抗原CD90。分离成单细胞后很快形成新的细胞球,并可分化出神经元与胶质细胞。结论:骨髓细胞可在体外诱导为能自我增殖的神经干细胞。
英文摘要:
  Objective:To investigate induction of neural stem cells from adult rat bone marrow in vitro.Method:Bone marrow cells were cultivated in serum-free medium with 20ng/ml basic fibroblast growth factor(bFGF) and epidermal growth factor(EGF).After cellular sph
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