李兵仓,李应玉,刘枚,赵刚,廖维宏.胎鼠脊髓对成鼠急性脊髓损伤的修复作用——光镜CB—HRP示踪与还原银染色[J].中国脊柱脊髓杂志,1992,(4):.
胎鼠脊髓对成鼠急性脊髓损伤的修复作用——光镜CB—HRP示踪与还原银染色
Function of fetal spinal cord on the repair of acute injured adult rats spinal cord: CB--HRP tracing and silver stain
  
DOI:
中文关键词:  脊髓损伤,脊髓移植,CB-HRP
英文关键词:Spinal cord injury,Transplantation,CB-HRP
基金项目:全军医药卫生青年科研基金题
李兵仓  李应玉  刘枚  赵刚  廖维宏
第三军医大学野战外科研究所 (李兵仓,李应玉,刘枚,赵刚)
,第三军医大学野战外科研究所(廖维宏)
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中文摘要:
  把E14胎鼠脊髓植入成鼠损伤脊髓后,分别在术后7、 15、 30、 45、 60、 120天,用 CB-HRP和还原银染色显示了宿主脊髓和移植物内的神经元及神经纤维。结果表明,虽然术后7天就可见宿主神经纤维再生进入移植物,但两种方法均不能显示移植的胚胎神经元及其突起。自术后30天起,移植物内神经元可被标志,但神经元的体积小,突起数目也短而少。随着神经元体积的逐渐增大和突起数目的不断增多延长,可见宿主神经元向移植物内投射突起,并和后者神经元相互联系。作者认为,胚胎脊髓虽可在术后早期机械修补成鼠脊髓损伤,但并不能与宿主脊髓组织发生广泛的有机纤维联系,这和胚胎脊髓移植后,神经元的缓慢分化发育有关。
英文摘要:
  After fetal spinal cord (E14) had been transplanted into adult rats spinal cord which had been hemisected and stired, neurons and nerve fibers of the host spinal cord and the grafts was studied by CB-HRP tracing and silver stain at day 7, 15, 30, 45, 60,
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